肠型胃腺癌及不典型增生组织中血管活性肠肽mRNA表达的研究

The expression of vasoactive intestinal peptide mRNA in gastric adenocarcinoma of intestinal type and dysplasia

目的 血管活性肠肽 (VIP)与肿瘤关系密切 ,VIP可促进和 /或抑制一些肿瘤生长 ,而且一些肿瘤细胞有VIP的自分泌调节作用。VIP对胃癌生长的影响 ,胃癌细胞是否分泌VIP及存在VIP自分泌调节作用尚有争议。研究肠型胃腺癌及不典型增生组织中VIPmRNA的表达情况 ,试图探讨VIP在胃腺癌发生发展中的作用。方法 通过RT PCR方法 ,检测 1 5例正常胃窦黏膜、1 6例不典型增生胃黏膜及 2 0例肠型胃腺癌组织中VIPmRNA的表达量 ,通过与恒定表达的β actin(内参照 )mRNA表达量比较 ,计算出VIPmRNA与 β actinmRNA表达量的积分光密度比值 (V/B) ,以V/B值反映各组织中VIPmRNA表达量的高低。结果 肠型胃腺癌组织中V/B值明显高于不典型增生胃黏膜及正常胃窦黏膜 ,其V/B值分别为 :1 .452 2± 0 .32 82 ,0 .962 3± 0 .2 2 5 0 ,0 .951 3± 0 .2 66 9,差异有非常显著性 (P<0 .0 1 )。正常胃窦黏膜与不典型增生胃黏膜中 ,V/B值的差异无显著性 (P >0 .0 5)。结论 肠型胃腺癌组织VIPmRNA表达量明显高于不典型增生胃黏膜 ;VIP可能在肠型胃腺癌的发生发展中起重要的作用。

Objective It has been known that vasoactive intestinal peptide (VIP) is closely related with the development of tumors; in particular it enhances and/or inhibits the growth of some tumors. There is a VIP autocrine regulation in some tumors, but the effect of VIP on the growth of gastric cancer has been argued. Whether gastric cancer cells secrete VIP or VIP autocrine regulation exists in the gastric cancer cells has not yet been reported. The purpose of the study was to observe the expression of VIP mRNA in gastric mucosa of patients with dysplasia and gastric adenocarcinoma tissues of intestinal type, and to evaluate the role of VIP in the pathogenesis of gastric adenocarcinoma. Methods Using RT PCR method, we detected the VIP mRNA expression in antral mucosa of 15 healthy controls, gastric mucosa of 16 patients with dysplasia and 20 patients with gastric adenocarcinoma of intestinal type. The ratio(V/B) of integrated optical density of VIP mRNA to that of β actin mRNA was calculated. The relative expression of VIP mRNA was represented by V/B. Results The V/B of gastric adenocarcinoma tissues of intestinal type was greater than that of gastric mucosa of patients with dysplasia and the antral mucosa of healthy controls( P <0.01), The former values of V/B were 1.452 2± 0.328 2,0.962 3±0.225 0, 0.951 3±0.266 9 respectively. There was no significant difference in V/B between healthy controls and patients with dysplasia( P >0.05). Conclusions The expression of VIP mRNA in the gastric adenocarcinoma of intestinal type was higher than that in dysplasia. VIP may play an important role in the pathogenesis and progress of gastric adenocarcinoma of intestinal type.