高效液相色谱法测定健脾平肝颗粒中芍药苷的含量

Determination of Paeoniflorin in Jianpipinggan Granules by HPLC

目的:为健脾平肝颗粒中芍药苷建立专属性含量测定方法。方法:采用HPLC法测定。色谱柱:YWG-C_(18),4.6mm×200mm(中科院大连化学物理研究所);流动相:乙腈-0.1％磷酸溶液(15:85);检测波长:230nm。结果:芍药苷的平均加样回收率为100.3％,RSD为2.0％(n=5)。结论:本法简便、快速,重复性好,为健脾平肝颗粒的质量控制和评价提供了可靠的依据。

Objective: To establish a special determining method for paeoniflorin in Jianpipinggan granules. Method: YWG-G18 column [4.6 mm(i. d.) × 200 mm] was used by HPLC, acetonitrile-water with 0.1% phosphoric acid as mobile phrase and detection wavelength at 230 nm. Result The average recovery of paeoniflorin were 100.3% with RSD 2.0%(n = 5). Conclusion: This method was proved to be simple, quick and reproducible, and provided a reliable basis for the quality control and evaluation of Jianpipinggan granules.