薄层扫描法测定贞芪扶正片中黄芪甲苷的含量

Content of Astragaloside IV in Zhenqifuzheng Tablet Determined by Dual Wavelength TLC Scanning

目的:制定贞芪扶正片中黄芪甲苷的含量测定方法。方法:采用薄层扫描法,以氯仿-甲醇-水(13:6:2)(10℃以下放置过夜)的下层溶液为展开剂,λ_s=530nm,λ_R=70nm,SX=3。结果:平均回收率为97.41％,r=0.9961,线性范围为1.0～6.0μg。结论:本方法可靠,数据准备,可作为贞芪扶正片中黄芪甲苷的含量测定方法。

Objective: To establish a method for determining astragaloside IV in Zhenqifuzheng tablet. Method: The content of astragaloside IV was determined by TLC scanning. The mobile phase was chloroform-methanol-water(13:6:2), of which the lower layer of solution, placed below 10℃, was werved as the developin agent; λs = 530 nm, λR = 700nm, SX=3. Result; The average recovery was 97.41% , r = 0.9961; A good linearity of astragaloside IV was in the range of 1.0-6.0 ug. Conclusion: The method is reliable and the date was accurate, and can be used for the quality control of Zhenqifuzheng tablet.