裸小鼠大肠癌肝脏微转移模型的建立及肿瘤细胞微转移的检测

Establishment of the model of hepatic micrometastasis of colorectal cancer in nude mice and detection of micrometastasis

目的 建立一种裸小鼠大肠癌肝脏微转移模型的方法。方法 运用盲肠造疝原位瘤块接种法建立裸小鼠的大肠癌模型 ,同时以Alu基因为标志 ,利用PCR法检测接种后不同时期的裸小鼠肝脏中发生微转移的肿瘤细胞。结果 盲肠原位接种瘤块后 2周 ,大部分裸小鼠均长出直径 1cm的盲肠实体瘤 ,接种成功率为 88%。接种后生长了四周以上的裸小鼠中有 5 7.1%在其肝组织中能够检测到Alu基因 ,而常规病理检查未发现异常 ,表明在这些裸小鼠的肝脏中存在着微转移的肿瘤细胞。结论 利用盲肠造疝瘤块原位接种法建立裸小鼠的大肠癌模型 ,其方法简单且成功率高 ,同时易于观察肿瘤的生长。大部分裸小鼠在接种后四周到六周就能够利用PCR法在肝脏中检测到微转移的肿瘤细胞。这一发现可为以后大肠癌微转移的基础研究提供一定的帮助。

Objective To study on the method of establishing the hepatic micrometastasis model of human colorectal cancer in nude mice.Methods The model of colorectal cancer in nude mice were established by the means of orthotopic transplantation with fresh neoplastic block into herniate cecum. Meanwhile, using Alu gene as a marker, hepatic micrometastasis of the nude mice were detected in different periods after transplantation by the means of PCR technique.Results Most nude mice got cecal carcinoma in 2 weeks after orthotopic transplantation with fresh neoplastic block into herniate cecum. Alu gene was detected in hepatic tissue in 57.1% of nude mice in 4 weeks after transplantation in which hepatic metastasis were not histopathologically detectable.Conclusion Establishment of colorectal cancer model in nude mice by means of orthotopic transplantation with fresh neoplastic block into herniate cecum is simple and with high success rate. Micrometastases can be detected in liver in most nude mice 4 weeks after transplantation using PCR technique.