T淋巴细胞核仁形成区嗜银蛋白(Ag-NORs)检测对恶性肿瘤临床意义的探讨

Nucleolar Organizer Regions associated proteins from peripheral T lymphocytes as a parameter of diagnosis and prognosis of malignant carcinoma patients

目的 探讨T淋巴细胞核仁形成区嗜银蛋白 (Ag NORs)在恶性肿瘤诊断及疗效观察中的临床价值。方法 制作银染T淋巴细胞涂片 ,显微镜下选取转化良好的T淋巴细胞 ,利用KL型肿瘤免疫图像分析系统检测其Ag NORs含量 (Ag NORs面积与细胞核面积的比值 ,即I /S % )。结果 检测 6 6例正常人、117例恶性肿瘤患者和 2 5例良性肿瘤患者 ,发现 :(1)正常人的T淋巴细胞Ag NORs含量与良性肿瘤患者和恶性肿瘤患者均有显著性差异 (P <0 .0 5 ) ,其均值分别为 :7.0 9± 0 .79、6 .31± 0 .75、5 .4 4± 1.13。 (2 )正常人和良性肿瘤患者的T淋巴细胞AgNORs含量与鼻咽癌、食管癌、乳腺癌、肝癌患者之间有显著性差异(P <0 .0 5 )。鼻咽癌、食管癌、乳腺癌、肝癌患者T淋巴细胞AgNORs检测的阳性率分别为 6 2 .86 %、71.4 3%、6 6 .6 7%、70 %。 (3)观察 6 7例鼻咽癌患者放疗过程的不同阶段 ,发现放疗中和放疗结束患者的T淋巴细胞Ag NORs均值分别为 4 .38± 1.16和 4 .16± 1.2 7,较放疗前 (均值 5 .5 5± 1.2 0 )低 ,但放疗一年后有所上升 (均值 5 .75± 0 .4 8)。 (4)观察 2 8例食管癌患者的不同治疗阶段 (术前、术后七天和术后一月 ) ,发现其T淋巴细胞AgNORs值没有显著性差异 (P >0 .0 5 ) ,分别为 :5 .2 3± 1.16、5 .11±

Objective To study the valuation of Nucleolar Organizer Regions associated proteins(Ag NORs) in peripheral T lymphocytes of malignant carcinoma patients.Methods Ag NORs was detected by KL cell image analysis system in 66 cases of normal volunteer, 25 cases of benign tumor patients and 122 cases of malignant carcinoma patients.Results (1) A lower Ag NORs area /nuclear area ratio (Ag NORs I /S%) in peripheral T lymphocytes was found in malignant carcinoma patients than that in benign tumor patients and the normal control. There was showing the statistically significant difference among the groups of normal control, benign tumor and malignant carcinoma ( P <0.05). (2) The groups of normal control and benign tumor also showed the significant difference with groups of nasopharyngeal carcinoma (NPC), esophagus carcinoma, breast carcinoma and hepatocellular carcinoma ( P <0.05); the positive rates of these malignant carcinomas were: 62.86%, 71.43%, 66.67%, 70.00% respectively. (3)The value of Ag NOR S were observed in 67 cases of NPC patients with radiotherapy. It showed that the average values during therapy and at end were 4.38±1.16 and 4.16±1.27 respectivaly, which were lower than that of pretherapy (5.55±1.20); but the value increased 1year after. (4) 28 cases of esophagus carcinoma patients were observed in three stage of therapy: pre surgery, seven days after surgery and a month after surgery. We found there was no significant difference in these three groups( P >0.05).Conclusion The percentage of Ag NORs I /S% in T lymphocytes could give the important information in tumor diagnosis and prognosis.