低压低氧对胎鼠海马神经元NMDA受体影响的实验研究

EFFECT OF FETUS HYPOBARIC HYPOXIA ON NMDA RECEPTOR OF HIPPOCAMPUS NEURONS

目的 :观察低压低氧环境对胎鼠海马神经元NMDA受体数目和通道特性的影响。方法 :采用原位杂交和膜片钳观察NMDA受体的数量和功能。结果 :胎鼠低压低氧后 ,NMDA受体数量和通道开放机率减少 ,通道开放时间常数减少 ,通道关闭时间常数增加。结论 :低压低氧影响到胎鼠NMDA受体的发育 。

Aim: To study the effect of fetus hypobaric hypoxia on the number and channel character of NMDA receptor of hippocampus neurons. Methods: Use in situ hybridization and patch clamp techniques. Results: After hypobaric hypoxia the number of NMDA mRNA positive neurons was decreased, the open probability of NMDA receptor was reduced, the open time constant was decreased,the close time constant was increased. Conclusion: Hypobaric hypoxia may change the development of NMDA receptor in fetus rat, then maybe effect learning and memory.