指数富集配基的系统进化原理及应用进展

Aptamers of Identification and Application in Systematic Evolution of Ligands by Exponential Enrichment Process

过去十几年 ,指数富集配基的系统进化 (SELEX)技术得到了充分的发展 ,目前已经成为在数量众多的靶分子中 ,高亲和性和特异性地确定适配子的一种崭新的方法 .SELEX的流程即是人工合成随机寡核苷酸序列、适配子筛选、扩增、再循环的过程 .在这一过程中 ,适配子修饰基团文库的应用使得SELEX流程更加经济、快捷、高通量 .回顾了SELEX的研究和应用 ,期待着这一方法能够在理论和实践上得到进一步的发展 .

In the past decade,systematic evolution of ligands by exponential enrichment (SELEX) technology has been under development,and is a general approach for identification of aptamers or oligonucleotide ligands that bind with high affinity and specificity to a wide rang of selected molecules on their versatility. The SELEX protocal is a process of synthesis random oligonucleotide sequents library pool, select special aptamers,amplify and iterative in vitro .Combinatorial modify groups libraries offer the convience in the SELEX process,making the screening process fast, easy and high throughput.Here the current designs and application of modified nucleotides for in SELEX process are reviewed, and expected to further the utility of this method in both practical and theoretical terms.