扣带回前部内脏伤害感受神经元的生物电活动

Biological Electric Activities of Visceral Nociceptive Neurons in Cat Anterior Cingulate Gyrus Revealed by Intracellular Recording Techniques

为了从神经元水平探讨大脑皮层内脏伤害感受的特性及机制 ,应用玻璃微电极细胞内电位记录技术 ,研究 18只猫扣带回前部 312个神经元的自发生物电活动 ,及其对电刺激同侧内脏大神经的诱发反应 .其中 ,82个为内脏伤害感受神经元 ,其自发生物电活动有 5种主要形式 .根据诱发反应的潜伏期等特性 ,内脏伤害感受神经元分为特异性内脏伤害感受神经元 (76个 ,92 6 8% )和非特异性内脏伤害感受神经元 (6个 ,7 32 % ) .内脏伤害性诱发反应分为兴奋性 (6 5 86 % )、抑制性 (17 0 7% )及混合性反应 (17 0 7% ) 3种 .结果提示内脏大神经的传入通路投射到同侧扣带回前部 ;扣带回前部神经元具有内脏伤害感受作用 ,存有特异性与非特异性内脏伤害感受神经元 。

To explore the cerebral cortex mechanism of visceral nociceptive sensation and its characteristics on the cell level with intracellular recording techniques,spontaneous biological electric activities and evoked responses of neurons in anterior cingulate gyrus(ACG) to stimulating ipsilateral greater splanchnic nerve(GSN) in 18 cats were investigated. Among 312 neurons,82 were visceral nociceptive neurons(VNNs),which mainly having five patterns of spontaneous biological electric activities. According to the characteristics of the evoked responses,VNNs were classified into specific visceral nociceptive neurons (SVNNs,92 68%) and non specific visceral nociceptive neurons(NSVNNs,7 32%). Modes of the evoked responses could be excitatory(65 86%),inhibitory(17 07%),or mixed ones(17 07%).The results suggest that ACG may be one of the representative areas of the ipsilateral GSN afferent pathway,and there exist two kinds of VNNs in ACG,which may be differently involved in pain modulation. The results may provide new experimental data for specific theory of pain.