Regeneration of Leaves in vitro in Cherry Rootstock Colt

樱桃砧木Colt离体叶片再生

Plants were regenerated from leaves of cherry rootstock Colt by two methods,the frequencies were 48 3% and 21 3%.Leaves were cultured on MS medium supplemented with NAA 1 0mg/L,KT 3 0mg/L and ZT 0 25mg/L.After 5～7 days leaves dedifferentiated,and formed callus.About 25 days later,callus grew into greenish or pink compact ones,the induction frequency was 100%.The color and structural feature of callus depended on the medium,culture condition and physiology phase of leaves.To induce callus,NAA was the main factor.Leaves rooted in medium only with NAA.In order to inhibit rooting and make callus grow fast,KT and ZT were added to the medium.Leaves cultured without light could quickly form callus during the initial stage of dedifferentiation.Young leaves dedifferentiated more easily than old leaves.After had been cultured about 50 days,callus were transferred to MS medium supplemented with NAA 0 2mg/L,IAA 0 5mg/L,6 BA 0 5mg/L,KT 1 0 mg/L and GA 0 5mg/L,and redifferentiated at a frequency of 21 3%.The callus of Colt leaves were difficult to redifferentiate.GA was important in the redifferentiation of callus.The result showed that callus could redifferentiate in all the mediums with GA,and could not redifferentiate without GA.The ability of redifferentiation of big callus was higher than that of small ones.And the physiology character of callus was also important.High regeneration frequency was obtained from greenish or pink compact callus.The frequency of regeneration of petioles was higher than that of leaves.Leaf petioles' regeneration need high concentration of cytokinin(more than 5 0mg/L).Leaf petioles were cultured on MS medium supplement with 6 BA 6 0mg/L,NAA 1 0mg/L and GA 0 5mg/L,and regenerated at a frequency of 48 3%.

以樱桃砧木Colt试管苗的叶片为外植体 ,通过先诱导愈伤组织分化不定芽以及叶片直接分化不定芽两种途径诱导再生。结果表明 :在MS附加NAA 1 0mg/L、KT3 0mg/L、ZT0 2 5mg/L培养基中 ,愈伤诱导率可达 1 0 0 % ;诱导的愈伤在MS附加NAA 0 2mg/L、IAA0 5mg/L、6 BA 0 5mg/L、KT 1 0mg/L、GA 0 5mg/L培养基中 ,不定芽分化率为 2 1 3% ;在MS附加 6 BA 6 0mg/L、NAA 1 0mg/L、GA 0 5mg/L中 ,叶片 -叶柄不定芽诱导率可达 48 3%。