摘要
建立了利用毛细管电泳简便、准确地测定白头翁汤中黄连和黄柏共煎生物碱煎出量的方法。采用自组装毛细管电泳装置,采用75μmi.d.×50cm弹性石英毛细管,以0 05mol/LNa2B4O7 CH3OH(体积比为85∶15)溶液作缓冲液,运行电压为14kV,检测波长为232nm。另外,通过实验优化了提取溶剂中乙醇的含量。实验结果表明:以小檗碱、巴马汀提取量为指标,30%(体积分数)的乙醇水溶液是提取白头翁汤中黄连和黄柏共煎生物碱的最佳溶剂。小檗碱和巴马汀的质量浓度分别在15 0mg/L~65 0mg/L、12 5mg/L~50 0mg/L时与其峰面积有良好的线性关系;小檗碱的平均回收率不低于95%,巴马汀的平均回收率不低于92%。
A rapid and accurate method for determining alkaloids from Coptis chinensis Franch. and Phellodendron amurense Rupr. decocted together in Baitouweng decocta was established. Experiments were carried out on a selfassembled capillary electrophoresis system. The experimental conditions were as follows: 75 μm id×50 cm fused silica capillary, 005 mol/L Na2B4O7CH3OH (85∶15,V/V) as buffer, applied voltage 14 kV and detection wavelength 232 nm. In addition, the concentration of ethanol in the extraction solvent was optimized. Experimental results showed that ethanolwater (30∶70, V/V) was the ideal solvent to extract the main effective ingredients from Coptis chinensis Franch. and Phellodendron amurense Rupr. decocted together in Baitouweng decocta. Berberine and palmatine had good linearities in the range of 150 mg/L-650 mg/L and 125 mg/L-500 mg/L respectively, and their average recoveries were 95.5%-104%(RSD 2.7%-6.9%) and 92.1%-103%(RSD 4.5%-6.7%) respectively.
出处
《色谱》
CAS
CSCD
北大核心
2002年第6期554-556,共3页
Chinese Journal of Chromatography