毛细管电泳法测定白头翁汤中黄连和黄柏共煎生物碱的煎出量

Determination of Alkaloids from Coptis chinensis Franch. and Phellodendron amurense Rupr. Decocted Together in Baitouweng Decocta by High Performance Capillary Electrophoresis

建立了利用毛细管电泳简便、准确地测定白头翁汤中黄连和黄柏共煎生物碱煎出量的方法。采用自组装毛细管电泳装置,采用75μmi.d.×50cm弹性石英毛细管,以0 05mol/LNa2B4O7 CH3OH(体积比为85∶15)溶液作缓冲液,运行电压为14kV,检测波长为232nm。另外,通过实验优化了提取溶剂中乙醇的含量。实验结果表明:以小檗碱、巴马汀提取量为指标,30%(体积分数)的乙醇水溶液是提取白头翁汤中黄连和黄柏共煎生物碱的最佳溶剂。小檗碱和巴马汀的质量浓度分别在15 0mg/L～65 0mg/L、12 5mg/L～50 0mg/L时与其峰面积有良好的线性关系;小檗碱的平均回收率不低于95%,巴马汀的平均回收率不低于92%。

A rapid and accurate method for determining alkaloids from Coptis chinensis Franch. and Phellodendron amurense Rupr. decocted together in Baitouweng decocta was established. Experiments were carried out on a selfassembled capillary electrophoresis system. The experimental conditions were as follows: 75 μm id×50 cm fused silica capillary, 005 mol/L Na2B4O7CH3OH (85∶15,V/V) as buffer, applied voltage 14 kV and detection wavelength 232 nm. In addition, the concentration of ethanol in the extraction solvent was optimized. Experimental results showed that ethanolwater (30∶70, V/V) was the ideal solvent to extract the main effective ingredients from Coptis chinensis Franch. and Phellodendron amurense Rupr. decocted together in Baitouweng decocta. Berberine and palmatine had good linearities in the range of 150 mg/L-650 mg/L and 125 mg/L-500 mg/L respectively, and their average recoveries were 95.5%-104%(RSD 2.7%-6.9%) and 92.1%-103%(RSD 4.5%-6.7%) respectively.