反相高效液相色谱法测定枳实、枳壳中橙皮甙和柚皮甙的含量

Determination of Hesperidin and Naringin in Fructus Aurantii Immaturus and Fructus Aurantii by Reversed-Phase High Performance Liquid Chromatography

采用高效液相色谱法测定了枳实和枳壳中的橙皮甙和柚皮甙含量。色谱柱为HypersilODS1柱(250mm×4 6mmi d ),流动相为乙腈 0 5%乙酸溶液(体积比为22∶78),流速为1 0mL/min,检测波长为283nm。柚皮甙和橙皮甙的线性范围分别为0 060g/L～0 604g/L和0 0125g/L～0 125g/L,柚皮甙和橙皮甙的平均回收率分别为97 1%和95 3%。该方法具有操作简便、分析快速、准确等优点。

An HPLC method was established for the determination of hesperidin and naringin in the Fructus Aurantii Immaturus and Fructus Aurantii from different places. Hesperidin and narigin were separated on a Hypersil ODS1 column with acetonitrile05% acetic acid (volume ratio of 22∶78) as the mobile phase and detected at 283 nm. The flow rate was 10 mL/min. The column temperature was set at 35 ℃. The calibration curves were linear in the ranges of 0060 g/L-0604 g/L and 0012?5 g/L-0125 g/L for naringin and hesperidin respectively. The average recoveries were 971% for naringin and 953% for hesperidin. The method is simple, rapid, accurate and reliable.