皱纹盘鲍肠上皮组织立克次体超微结构与细胞病理学研究

Study on the Ultrastructure of Rickettsia and theCell Pathology of the Intestinal Epithelial Tissue ofHaliotis Discus hannai Ino

利用电镜技术对患病濒死皱纹盘鲍 (H aliotis Discus hanna Ino)进行病原体检测和病理观察 ,在肠上皮组织中发现大量立克次体。研究表明 ,皱纹盘鲍肠上皮细胞中的立克次体分为增殖型立克次体和静止型立克次体 2种类型。立克次体以皱纹盘鲍肠上皮细胞为感染的靶细胞 ,造成细胞微绒毛全部或部分破坏 ,细胞功能丧失 ,是造成养殖皱纹盘鲍病害发生和死亡的主要病原体。

The Rickettsia in the enteric epithelial tissue of the diseased Haliotis Discus hannai Ino were observed with an electron microscope. The studies showed that the Rickettsia had two forms: the Vegetative and the Vesting form. The Vegetative form txisted in many shapes: rotundity, ellipse, dumbbell, longstick, curve, etc.The diameter ranged from 0.14 to 0.27 μm. This form of Rickettsia had an integrated cell membrane and a nucleoid with a low electron density. It was reproduced by division and by budding. While the Rickettsia in Vesting form was small and had a medium electron density and in the shape of round or short sticks, with a diameter from 0.07 to 0.1 μm. It was derived from the Vegelative form by budding. The target cell of the Rickettsia was the Intestinal Epithelial cell. The whole or part of the microvilli of the epithelial cell was destroyed and the cell was deprived of its function. It was a main pathogen which caused disease and death of Haliotis Discus hannai Ino.