纤维素热解液发酵制乙醇

Fermentation of Cellulosic Pyrolysate for Producing Ethanol

纤维素热解液中的主要产物是1,6-脱水-b-D-吡喃葡萄糖(简称内醚糖),通过催化水解内醚糖和脱毒处理,热解液可直接发酵产乙醇. 研究结果表明,在121oC和0.2 MPa条件下的多种催化处理中,0.2 mol/L硫酸处理的热解液获得的葡萄糖产量最高,达17.35%. 水解后含葡萄糖的热解液经Ca(OH)2中和、硅藻土吸附处理,能被Candida krusei YZ-1发酵为乙醇,产率为0.42 g/g(乙醇/葡萄糖), 达理论产率的84%. 而Zymomonas mobilis对于各种处理液的发酵结果都很差.

The cellulosic pyrolysate mainly containing 1,6-anhydro-b-D-glucopyranose (levoglucosan) could be hydrolyzed to glucose and further fermented to ethanol with microorganisms. Among the various hydrolysis treatments, the highest glucose yield (17.35%) was obtained by 0.2 mol/L H2SO4 at 121oC for 20 min. Such hydrolysate treated by neutralization with Ca(OH)2 and absorption with diatomite for detoxification was used for fermentation to ethanol by Candida krusei YZ-1. The yield was 0.42 g/g (ethanol/glucose), up to 84% of the theoretical yield. Zymomonas mobilis performed very poorly in fermenting all treated pyrolyate to ethanol.