摘要
马铃薯两个品系小叶子 x 多子白和乌盟601的叶肉原生质体在原生质体培养基中诱导出愈伤组织。叶肉原生质体来源的愈伤组织转移到 MS+2mg/l ZT+0.1mg/l IAA 培养基中,培养至70天以后,开始发生芽的分化。待芽生长到2—3cm 高度时,转入 MS+0.05mg/lNAA 培养基中,很快出根长成完整植株。带1—2片叶的茎段移栽入灭菌的混合土壤中生长并结出薯块。
Protoplasts from potato mesophyll of two strains (Solannum tuberosum L.cv.Xiao Yie Zi x Duo Zi Bia and Solanum tuberosum L.cv.Wu Meng 601) were induced to callus in culture medium of protoplasts.The callus derived from mesophyll protoplasts were transferred to MS medium with 2 mg/l ZT+0.1 mg/l IAA.Shoots regenerated from the callus were detected after 70 days of culture.The shoots which had grown to a height of 2—3 cm were transfer- red to MS medium with 0.05 mg/l NAA.Roots were coming out in a few days.Complete plantlets were achieved.Stem segments with 1—2 leaves were then transferred to a mixture of sterilized soil and grown,and produced tuber.
