摘要
本文用聚丙烯酰胺凝胶电泳测定了柑桔近缘植物14个种群的种子及幼苗的酯酶同工酶。根据酶谱及扫描图的异同,分析了彼此的亲缘关系。试验结果表明,柑桔近缘植物种属间的酯酶同工酶的酶带数目、酶活性、迁移率及酶谱扫描均有不同程度的差异。同一品种不同发育时期的同工酶也具有不同表现形式。特别是柑桔种子的酯酶同工酶谱一般较稳定,可以作为柑桔亲缘关系的生化遗传指标。
In this experiment,the polyacrylamide gel electrophoresis is adopted to test the esterase isozyme of seeds and seedling of the fourteen species of related Citrus plants.The relationship with each other is analysed according to the resutls of zymogram and scanning chart.The data show that there are differences of various degrees in the number of the isozyme bands,the enzyme activity,the migration of enzymes and their chromatogram scanning.The isozyme of the same species has different forms of expression in the different period of development. The esterase isozyme zymogram of the Citrus seed is generally,stable.It can be used as the biochemical and genetical index to detemine the genetic ralationship of Citrus.
