摘要
在花粉母细胞减数第一分裂时期,对多花菜豆(Phaseolus coccineus L.)4个品种、菜豆(P.vulgaris L.)5个品种和菜豆变种[P.vulgaris var.aborigineus (Burk.)baudet]的一个收集样品的二价体次级联会(次级配对)进行了细胞学检查,同时也提供了一种评价观测到的联会程度和预期的随机联会程度之间的偏差统计学方法。发现次级配对的程度是高度显著的。对透射电子显微技术加以改进,并使之能够观察压片完整的花粉母细胞,显示出在次级联会的双价体之间发生有形的联结。
Four cultivars of Phaseohts coccineus L,five cultivars of P.vulgaris L.and one collection of P.vulgaris vat.aborigineus (Burk.)Baudet were examined cytologically for the presence of secondary association of bivalents (bivalent pairing) during metaphase I of meiosis in pol- len mother cells.Statistical methods were presented for evaluating the deviation of the ob- served degree of association to that expected at random.The degree of secondary pairing was found to be highly significam.Transmission electron microscope techniques were modified tc enable the viewing of squashed but intact pollen mother cells which showed that a physical connection could occur between bivalents secondarily associated.
