摘要
本文介绍了一种简单可行的,用以观察和鉴定一种植物细胞壁蛋白质——富含羟脯氨酸糖蛋白(HRGP)的方法。胡萝卜 HRGP 属于一种伸展蛋白(extensin)。通过简化的装置将HRGP 喷射到云母片上,再经旋转投影,使其在透射电子显微镜下呈现为棒状分子(rod-likemolecule)。经电镜观察和测量表明,HRGP 的分子长度为87nm。
This paper describes a method for observing and identifying the cell wall protein—— hydroxyproline-rich glycoprotein(HRGP)which has been characterized as an extensin.The HRGP prepared from carrot callus was sprayed on a mica sheet by using a simplyzed spra- ying device.After rotatory shadowing,HRGP was observed under electron microscope and it appeared as a rod-like molecule.Measurement showed that the molecular length of HRGP was about 87 nm.
