摘要
以成熟种子为外植体,在离体培养中研究了体细胞胚胎发生。组织学研究表明,愈伤组织是由成熟种子盾片的表皮吸收细胞首先产生的,随后从愈伤组织分化出胚状体。象水稻合子胚的结构一样,体细胞胚状体主要由盾片、胚芽稍及胚根组成。在胚状体内可以观察到不同发育时期的原胚,包括大量的单一的胚性细胞、二细胞、四细胞及分化程度不同的原胚以及一些畸形胚状体。从本实验可以初步认为由籼稻体细胞培养而获得的胚状体在结构上也有象合子胚一样的基本结构,并且是单细胞起源的。
The somatic embryogenesis was established from mature dehulled seeds.The histological research showed that embryogenic calli were initiated first from absorbed cells of scutellum of mature seed.And then the embryoids derived from the surface of embryogenic callus.Ha- ving been the same structure like a zygotic embryo of rice,the embryoids possessed the ma- jour parts of scutellum,coleoptile and coleorhiza.In an embryoid,several developmental stages of pro-embryoid,including single embryogenic cells,two-,four- and multiple cell stage pro-embryoids and some abnormal embryoids were observed.It could be concluded from this experiment that the embryoid from somatic cell culture in Indica rice possessed an original form of a plant in structure like a zygotic did and derived from a single cell.
