摘要
BTV HbC株和蓝舌病毒标准株BTV 10分别接种在不同种系细胞如猴肾传代细胞 (Vero)、人宫颈癌细胞(Hela)和小鼠神经胶质瘤细胞 (C6)等细胞株上 ,比较研究了BTV HbC在不同种系细胞上的增殖特征 ,BTV HbC与BTV 10在相同细胞上的复制增殖特征 ,病毒与细胞相互作用的显微和超微结构特征。用免疫交叉反应研究了BTV HbC株与BTV 10型标准株之间的血清学关系。本研究结合本室对BTV HbC株基因组图谱分析和蓝舌病毒群特异性抗原编码基因S7的RT PCR分析 ,进一步证实了BTV
Bluetongue virus\%(BTV)HbC strain and BTV 10 (the international standard strain) were respectively inoculated onto the different species monolayer cells such as Vero cell (monkey renal cell), C\-6 cell (mouse neuroglioma cell) and Hela cell (human cervical cancer cell). We studied comparatively the replicated characteristics of BTV HbC in different species cells, with BTV 10 in the same cells, morphologic characteristics under microscopy and electron microscopy after interaction between BTV HbC and different cells, the serologic relation between BTV HbC and BTV 10. It is further proved that the BTV HbC strain is probably a new type of Bluetongue virus when connecting our previous research about BTV HbC genome map and RT PCR analyses of BTV HbC S\-7 gene coding group specific antigen.
出处
《中国病毒学》
CSCD
2002年第4期362-366,共5页
Virologica Sinica
基金
国家自然科学基金 (3 92 70 0 3 )
