绿草履虫-小球藻共生系统中共生藻对宿主细胞超微结构的影响

The Ultrastructural Effect of Symbiotic Chlorella on its Host in Paramecium bursaria-Chlorella System

应用透射电镜术显示了含小球藻绿草履虫和人工诱导获得的无小球藻绿草履虫细胞的超微结构特征。无小球藻绿草履虫细胞内有大量处于不同消化阶段的食物泡及膜性小泡 ,在细胞质内常见有线粒体聚集分布以及内质网分布其中 ,细胞大核内核仁数目增多 ,并聚集形成多个核仁区。含小球藻绿草履虫中细胞膜性结构较少见 ,细胞大核中核仁数目较少。结果表明 ,小球藻共生体可能影响了宿主草履虫细胞中所述细胞器的功能、数量和分布 ,并影响了核仁的功能、数量和分布。

The fine structure of Chlorella \|bearing Paramecium bursaria and Chlorella \|free Paramecium bursaria were studied with transmission electron microscopy. In Chlorella \|free P.bursaria, a large amount of food vacuoles and membrane vacuoles in different digestive process were observed. Compared with Chlollera \|bearing P.bursaria, Chlorella \|free P.bursaria had a greater quantity of cytoplasmic mitochondria and endoplasmic reticulum, most of which was accumulated in certain regions. In the macronucleus of Chlorella \|free P.bursaria there were many round nucleoli while few nucleoli could be observed in the macronucleus of Chlorella \|bearing P.bursaria. This suggests that the existence of the Chlorella symbiont in P.bursaria may affect the function, quantity, and location of the previously mentioned organelles in host cells, as well as the nucleoli.