地鼠肾细胞培养的CTN株狂犬病新疫苗研究

Study on a new rabies vaccine prepared from CTN strain virus propagated in primary hamster kidney cell culture

应用细胞毒种代替豚鼠脑毒种制备狂犬病地鼠肾细胞纯化疫苗。将狂犬病毒CTN株在原代地鼠肾细胞(PHKC)传代适应 ,用病毒培养液上清作为生产用毒种 ,结果通过在PHKC传 10多代 ,适应后病毒滴度达到 7.0LogLD50 /ml,并应用适应株 (CTN LS HK)细胞毒种制备三批疫苗 ,其效力在 6 .11～ 6 .5 5IU/ml,高于用aG株豚鼠脑毒种制备的三批疫苗效力 (3.77～ 5 .85IU/ml)。

The development of a new rabies vaccine prepared from CTN strain virus propagated in primary hamster kidney cell (PHKC) culture was performed. The virus seed of CTN was serially passaged in PHKC for adaptation and the rabies vaccine was prepared by using infected cell culture as virus seed.After more than tenth passages the CTN virus was adapted in PHKC with a virus titer around 7.0LogLD 50/ml. The potencies of three lots of purified vaccine made by the adapted virus (CTN-LS-HK)were 6.11～6.55IU/ml that appeared higher than the vaccines made by aG strain of rabies seed virus grown in guinea-pigs brain (3.77～5.85 IU/ml). The CTN adapted virus strain can be used for production of a more immunogenic and safer rabies vaccine, which doesn't contain brain tissue and will avoid the contamination of extraneous agents in the guinea-pigs.