重组人乳头瘤病毒核酸疫苗诱导特异性淋巴细胞增殖

Study on Recombinant HPV DNA Vaccine Inducing Specific Lymphoproliferation

采用分子克隆技术 ,将人乳头瘤病毒 16型E7(HPV 16E7)基因重组于真核表达载体上 ,构建了HPV 16E7核酸疫苗。将该疫苗通过皮内注射方式免疫Balb/c纯系小鼠。基因免疫后制备小鼠脾淋巴细胞悬液 ,经体外E7蛋白再次刺激后用MTT比色法检测出了特异性淋巴细胞增殖反应。由于特异性淋巴细胞增殖是反映细胞免疫功能的简便有效方法 ,所以本实验表明HPV 16E7核酸疫苗构建正确 。

Using molecular cloning technique,HPV16 E7 gene was cloned into eukarytic expression vector.HPV16 E7 DNA vaccine was constructed and Balb/c mice were immunized by intradermal administration with HPV16 E7 vaccine.After immunization,mice spleen lymphocytes were prepared and restimulated by E7 protein \%in vitro\%. Specific lympproliferation were detected by MTT colorimetric assay.Because detection of specific lymphoproliferation is a simple and efficient way,which can reflect cell mediated immunity.The study showed that the construction of HPV16 E7 vaccine was correct.It can induce a specific cell mediated immunization.