菌-藻相互作用下胞外酶活性变化研究

THE VARIATION OF BACTERIAL EXTRACELLULAR EN-ZYMATIC ACTIVITY UNDER THE INTERACTION BE-TWEEN BACTERIA AND ALGAE

报道了在实验室培养条件下塔马亚历山大藻 (Alexandriumtamarense)与几株海洋细菌之间的生态关系。实验表明 ,在培养前期 ,培养液中的营养成分尚足以维持藻类和细菌的生长 ,细菌的加入是胞外酶活性升高的主要原因所在 ;在培养的后期 ,混合培养液和藻对照培养液中胞外酶活性持续升高。A.tamarense在单独培养下 ,其胞外酶活性从第12天起才有明显的提高 ,第16天增加较快,达到0.104μmol/(L·h);至第20天藻体衰败时 ,胞外酶活性达到最高水平 ,为0.385μmol/(L·h)。Z7 细菌单独培养时 ,其胞外酶活性在单独培养的2d后有一个高峰值0.133μmol/(L·h) ,随着培养时间的延长 ,其酶活性逐渐降低。细菌Z7 和Z10 与A.tamarense共培养下 ,两者胞外酶活性变化趋势相似。

This article reported the ecological relationship between Alexandrium tamarense and several strains of marine bacteria under laboratory cultivation condition.The result showed that at the prophase of cultivation,the addition of bacteria con- duced an increasing ofβ-glucosidase activity(βGlcA)when the nutrition in the culture medium was sufficient to maintain the growth of algae and bacteria.At the anaphase of cultivation,theβGlcA rose continually in the mixed cultivation and the control.Also the finding indicated that firstly,after12 th day in the pure culture of A.tamarense,theβGlcA rose obviously and increased to0.104mmol/(L·h)at the16 th day.Secondly,the highest level of theβGlcA(0.385mmol/L·h)has been detected at the20 th day during the decline of algae.And finally when Z 7 bacteria was cultivated sepa-rately,theβGlcA reached a peak value at2 nd day(0.133mmol/(L·h)),then begun decreasing with the time.Moreover,the variation ofβGlcA,when Z 7 and Z 10 were cultured with algae respectively,has been investigated during this research work.when