摘要
将鹅细小病毒(GPV)和番鸭细小病毒(MPV)主要结构蛋白(VP2-VP3)基因克隆到核酸疫苗质粒pIRES1neo载体上,构建了核酸疫苗重组质粒pIGVP1和pIMVP,通过脂质体转染法分别将重组质粒转染到鹅胚成纤维细胞和番鸭胚成纤维细胞中,核酸疫苗重组质粒pIGVP1和pIMVP分别转染鹅胚成纤维细胞和番鸭成纤维细胞中,于转染后72h收取细胞,细胞裂解液裂解后,经Westernblot检测其表达产物可出现特异性反应带,证明表达产物具有很好的反应原性。
Two eukaryotic expression vector systems,pIGVP1and pIMVP,were constructed by cloning the VP2-VP3gene of GPV and MPV into the downstream sequences of viral promoter of pIRES1neo vector.The recombinant plasmids were selected and identified by restriction enzyme digestion test,then transfected into the goose embryo fibroblast cells(GEF)and the muscovy duck embryo fibroblast cell(MDEF)respectively.As the culture being harvested after3days,the recombinant proteins were successfully detected by Western blot.
出处
《生物技术通讯》
CAS
2002年第6期433-435,共3页
Letters in Biotechnology
基金
国家自然科学基金(39870557)
教育部和总后勤部回国人员启动基金(98H026)
