肝细胞癌中表皮生长因子与血管内皮生长因子过量表达的关系

Correlation between epidermal growth factor and overexpression of vascular endothelial growth factor in hepatocellular carcinoma

目的 研究肝细胞癌 (HCC)中表皮生长因子 (EGF)与血管内皮生长因子 (VEGF)过量表达的关系。方法 通过免疫组化SABC法 ,检测 36例HCC组织及其相应癌旁肝组织和 6例正常肝组织中EGF、VEGF和微血管密度的表达 ,并对这些指标进行相关分析。离体实验中 ,用重组人EGF刺激人肝癌细胞系HepG2 ,采用半定量逆转录PCR检测VEGF的表达情况。结果 36例HCC组织中 ,EGF和VEGF表达阳性率分别为 75 .0 %和 88.9%。Spearman等级相关分析显示 ,HCC组织中EGF的表达与VEGF的表达具有明显的正相关关系 (r=0 .4 6 2 ,P <0 .0 1)。重组人EGF可以以浓度和时间依赖性的方式诱导HepG2 细胞中VEGF的转录。结论 HCC中EGF的表达是VEGF过量表达的基本原因之一。

Objective To investigate the correlation between epidermal growth factor (EGF) and overexpression of vascular endothelial growth factor (VEGF) in hepatocellular carcinoma (HCC). Methods Expressions of EGF, VEGF and microvessel density were studied through immunohistochemical SABC method in 36 HCC specimens, their paraneoplastic liver tissues and 6 normal liver tissues with the correlation between these parameters analyzed. Recombinant human EGF was used to stimulate HepG_2 cells and semi-quantitative reverse transcription PCR was adopted to detect the expression of VEGF in HepG_2 cells. Results The positive rates of EGF and VEGF expression in HCC tissue were 75.0% and 88.9%. There was positive correlation between EGF and VEGF expression (P<0.01, r=0.462). Recombinant human EGF could induce the expression of VEGF in HepG_2 cells in a dose and time dependent manner. Conclusion The expression of EGF in HCC underlies the overexpression of VEGF in HCC.