瘢痕疙瘩周围皮肤成纤维细胞分布特征及其增殖活性

The distributing characteristics and proliferating activity of fibroblasts from the surrounding skin of keloids

目的 初步探讨瘢痕疙瘩周围皮肤中是否有异常成纤维细胞存在 ,以期为临床提供一个更为准确的治疗范围。方法 采用体外培养成纤维细胞技术比较瘢痕疙瘩及其周围皮肤与正常皮肤成纤维细胞的形态及分布特征 ,用MTT比色法比较其细胞增殖活性。结果 瘢痕疙瘩周围皮肤 (距边缘 0 5cm范围内 )成纤维细胞形态与正常皮肤一致 ,但在其散在分布区域存在交错重叠生长现象 ,与瘢痕疙瘩相似。瘢痕疙瘩周围皮肤成纤维细胞增殖活性与瘢痕疙瘩中央部相似 ,介于瘢痕疙瘩边缘部与正常皮肤之间。结论 瘢痕疙瘩周围皮肤中存在生物活性异常的成纤维细胞。

Objective To investigate whether there are abnormal fibroblasts derived from the surrounding skin of keloids so that a more accurate therapy for keloids could be obtained. Methods Samples were taken for cell culture. When primary cells fully covered the culture bottle, the shape and distributing characteristics of fibroblasts were observed under the light microscope. 6-8 passage fibroblasts were selected for comparing the proliferating activity by MTT contrasting color method. Results The fibroblasts have the same shape in all groups. But the fibroblasts derived from the surrounding skin grow crossly and overlapped just as the fibroblasts from keloids. The proliferating activity of the fibroblasts from surrounding skin is not as high as that from the border of keloids, but is higher than the normal skin fibroblasts derived either from a normal person or a patient with keloid. Conclusion It is likely that there are abnormal fibroblasts in the surrounding skin of keloids.