完全肠道外营养添加维生素A、E、C对腹腔感染后抗氧化能力的影响

Influence of vitamin A,E,C-supplemented TPN on the antioxidation state in intraperitoneal sepsis

目的 :观察完全肠道外营养 (TPN)添加维生素A、E、C对腹腔感染后抗氧化能力的影响。 方法 :采用盲肠结扎加穿孔 (CLP)模型 ,将SD大鼠随机分为对照组 (CLP +TPN)和治疗组 (CLP +TPN +维生素A、E、C) ,于 0、1、3、5天观察血浆及肝组织中维生素含量、丙二醛 (MDA)、还原型谷胱甘肽 (GSH)的变化。 结果 :治疗组维生素A、E、C及GSH显著高于对照组 ,MDA明显低于对照组。 结论 :TPN添加维生素A、E、C能改善腹腔感染后机体的抗氧化能力 。

Objectives:To determine the impact of vitamin A,E,C supplemented TPN on the antioxidative state in intraperitoneal sepsis. Methods:A rat model of intraperitoneal sepsis was made by cecal ligation and puncture(CLP). Healthy male SD rats were divided into control group(CLP+TPN) and treatment group(CLP+TPN+Vitamin A,E,and C). Levels of VA, VE, VC, MDA and GSH in the plasma and liver were determined. Results:Increased levels of MDA were found in the control group. VA, VE, VC and GSH in the plasma and liver were higher in the treatment group than the control group, MDA was lower in the treatment group than the control group, and favorable outcome was found in the treatment group. Conclusions:Vitamin A?E?C supplemented TPN could ameliorate antioxidative ability effectively in intraperitoneal sepsis.