微生态制剂对异育银鲫消化酶活性的影响

Effects of dietary Bacillus sp. and selenoyeast on activities of digestive enzymes of allogynogenetic crucian carp

在基础饲料的基础上,分别添加0.1%、0.2%、0.4%芽孢杆菌,0.3%、0.6%、1.2%硒酵母,混合添加0.2%芽孢杆菌和0.3%、0.6%、1.2%硒酵母,制成9种微生态制剂饲料,以基础饲料为对照,在水泥池中饲养异育银鲫60d。然后,对异育银鲫肠道和肝胰脏的蛋白酶、淀粉酶进行了测定。结果表明,添加0.2%芽孢杆菌,异育银鲫肠道、肝胰脏蛋白酶活性分别比对照组提高466.2%和85.4%,添加0.1%芽孢杆菌,肠道肝胰脏淀粉酶活性分别比对照组提高83.7%和129.5%;添加硒酵母对肠道蛋白酶活性无显著影响,但添加0.6%硒酵母能使肝胰脏蛋白酶活性比对照组提高43.7%,添加0.3%硒酵母能使肠道、肝胰脏淀粉酶活性分别比对照组提高84.2%和163.9%;同时添加0.2%芽孢杆菌和0.3%硒酵母能使肠道蛋白酶淀粉酶活性分别比对照组提高395.5%和95.4%,同时添加0.2%芽孢杆菌和0.3%、0.6%、1.2%硒酵母对肠、肝胰脏蛋白酶淀粉酶活性无显著影响。与单独添加0.2%芽孢杆菌相比,同时添加0.2%芽孢杆菌和硒酵母肠道、肝胰脏蛋白酶活性随硒酵母添加量的增加而显著降低,但淀粉酶活性两者之间差异不显著;与单独添加硒酵母相比,0.2%芽孢杆菌和硒酵母同时添加对肠道、肝胰脏蛋白酶、淀粉酶活性促进作用并不显著。

Allogynogemetic crucian carp (average initial weight,4.2±0.3g) were fed with basal diet supplemented 0.1%,0.2%,0.4% Bacillus sp.,0.3%,0.6%,1.2% selenoyeast, complex of 0.2% B.sp. and 0.3%,0.6%,1.2% selenoyeast respectively for two months. Control group was fed with basal diet.Triplicate groups were made for each diet. The activities of protease and amylase in the intestine and hepatopancreas were determined. The activities of protease in the intestine and hepatopancreas fed with dietary 0.2% B.sp. were 466.2%and 85.4% higher respectively than those of the control group.The activities of amylase in the intestine and hepatopancreas fed with dietary 0.1% B. sp. were 83.7% and 129.5% higher respectively than those of the control group. The activities of protease in the intestine with three levels of selenoyeast were similar to those of the control group. The activity of protease in the hepatopancreas with dietary 0.6% selenoyeast was 43.7% higher than that of the control group, the activities of amylase in the intestine and hepatopancreas with dietary 0.3% selenoyeast increased by 84.2% and 163.9% respectively. The activities of protease and amylase in the intestine fed with dietary 0.2% B. sp.and 0.3% selenoyeast complex were 395.5% and 95.4% higher respectively than those of the control group, while the activities of protease and amylase in the hepatopancreas fed with dietary 0.2% B. sp. and three levels of selenoyeast were not remarkably higher than those of the control group. Compared to dietary 0.2% B. sp. group, the activities of protease in the intestine and hepatopancreas fed with dietary 0.2% B. sp. and three levels of selenoyeast complex decreased drastically with the increasement of the level of dietary selenoyeast, while there were no remarkable difference in the activities of amylase between two groups. Compared to dietary selenoyeast group, the activities of protease and amylase in the intestine and hepatopancreas fed with dietary selenoyeast and 0.2% B. sp. complex were not changed remarkably.