郑州与北京地区HCV5′-非编码区酶切基因分型对比研究

Study on HCV Genotypes in Anti-HCV Positive Blood Donors in Zhengzhou and Beijing Areas

目的 探讨郑州与北京地区丙型肝炎病毒 (HCV)基因型分布情况。方法 分别对郑州地区的 90例抗 -HCV阳性献血员及北京地区 2 6 9例肝炎患者血清采用逆转录套式聚合酶链反应 (RT- nested- PCR)进行 HCV RNA检测 ,其中郑州地区 80例呈 HCV RNA阳性 ,北京地区 5 6例呈 HCV RNA阳性 ,并对两地区病例的阳性 PCR产物进行5′-非编码区 (5′- NCR)酶切基因分型。结果 郑州地区 HCV 型感染 5 5例 (6 8.8% ) ,HCV 型感染 2 1例 (2 6 .2 % ) ,HCV / 型混合感染 4例 (5 .0 % )。北京地区 HCV 型感染 4 9例 (87.5 % ) ,HCV 型感染 7例 (12 .5 % ) ,无 HCV / 型混合感染。结论 HCV 型感染是郑州地区的优势株 ,其次是 HCV 型 , / 型混合感染较少见。而北京地区主要以 HCV 型为主 ,HCV 型感染少见。

Objective To study the genotypes of HCV in Zhengzhou and Beijing areas.Methods HCV RNAs were detected by reverse transcription nested polymerase chain reaction (RT nested PCR) in 90 anti HCV positive blood donors in Zhengzhou and 269 patients with hepatitis in Beijing .The PCR products of 80 HCV RNA positive sera in blood donors and 56 HCV RNA positive sera in patients with hepatitis were analysed by restriction fragment length polymorphism.Results For Zhengshou area, 55(68.8%) were HCV type Ⅱ infection, 21(26.2%) were type Ⅲ infection, and 4(5.0%) were type Ⅱ/Ⅲ mixed infection , while for Beijing area,49(87.5%) were HCV type Ⅱ infection, 7(12.5%) were type Ⅲ infection.Conclusion HCVⅡ infection was dominant followed by HCVⅢ and mixed infections in Zhengzhou areas ,while in Beijing area HCV Ⅱ infection was dominant.