组胺在紫外线上调纤溶活性中的作用研究

A study of the role of histamine in the up-regulation of fibrinolytic activity activated by full-spectrum ultraviolet irradiation

目的 探讨组胺在紫外线 (ultraviolet ,UV)激活纤溶系统 (fibrinolyticsystem ,FS)效应中的作用。方法 将 14只健康成年西北杂种犬随机分成 3组 :UV组和UV +bufrolin组 (各 5只犬 ) ,行全光谱UV2 0cm× 2 0cm ,44 7.72mJ/cm2 照射胸腹部 1次 ;UV +bufrolin组对UV照射区皮肤预先局部给予bufrolin(组胺释放抑制剂 ) ;空白组 (4只犬 )不行UV照射 ,作对照用。观察 3组UV照射后 48h内照射区皮肤组织内组胺水平及血纤溶酶原激活物 (plasminogenactivator ,PA)活性的动态变化。结果 UV组在UV照射后 3h血PA活性明显增高 ,照后 9h达其峰值 ,照射区皮肤组胺浓度在UV照射后 9h明显升高 ,其峰值约在UV照射后 15h ;局部皮肤预先给予bufrolin后 ,UV +bufrolin组UV照射区皮肤组织内组胺浓度升高不明显 ,血PA活性仍明显增高。

Objective To study the possible role of histamine in the up-regulation of fibrinolytic system(FS) activated by full-spectrum ultraviolet(UV) irradiation. Methods Fourteen normal healthy adult dogs were randomly divided into 3 groups.The dogs in the UV group ( n =5) were treated by single 20 cm×20 cm, 447.72 mJ/cm 2 full spectrum UV irradiation on the thoracoabdominal skins, while those in the UV+bufrolin group ( n =5) was administered, by means of iontophresis, with bufrolin into the local skin to be irradiated immediately before UV irradiation, and those in the control group( n =4) got neither UV irradiation nor bufrolin treatment. The histamine concentration in the UV irradiated skin and plasminogen activator(PA) activity in plasma were tested dynamically during 48 h postirradiation . Results The plasma PA activity in the UV group was significantly increased at 3 h postirradiation( P <0.01),with its peak at 9 h after irradiation; histamine levels in suction blisters markedly increased at 9 h postirradiation( P <0.01), with its peak at 15 h after irradiation, and no significant relationship between the histamine level and PA activity was noted( P >0.05). In the UV+bufrolin group, the timedependent changes of plasma PA activity had the same characteristic as that in the UV group( P >0.05), although histamine level was not significantly changed because of bufrolin administration before the UV irradiation. Conclusion Histamine might not be invovled in the up-regulation of fibrinolytic activity caused by full spectrum UV irradiation.