中药灌肠对实验性炎症性肠病T淋巴细胞粘附分子表达的影响

Effect of Chinese Herb Enema on Expressions of T Lymphocyte Adhesion Molecule in Experimental Inflammatory Bowel Disease

目的 通过制作实验性炎症性肠病 (inflammatoryboweldisease,IBD)的动物模型来探讨IBD的发病机制并观察中药灌肠治疗后对其T淋巴细胞及其粘附分子表达的影响。方法 采用免疫方法制作动物模型 (30只 )后分为 3组 :Ⅰ组 (中药组 10只 ) ,Ⅱ组 (柳氮磺胺嘧啶即SASP组 10只 ) ,Ⅲ组 (模型对照组 10只 ) ,应用细胞分子学方法检测LTR、IL 2、T淋巴细胞亚群CD4+ 、CD8+ 及其粘附分子CD4+ CD11a+ 、CD4+ CD18+ 、CD8+ CD11a+ 、CD8+ CD18+ 表达的变化。结果 Ⅲ组LTR、IL 2、CD8、CD8+ CD11a+ 、CD8+ CD18较正常组低 (P <0 .0 1) ,CD4+ 、CD4+ CD11a+ 、CD4+CD18无明显变化 (P >0 .0 5 )。经治疗 ,Ⅰ组、Ⅱ组能有效提高功能低下的LTR、IL 2、CD8+ 、CD8+ CD11a+ 、CD8+ CD18水平 ,与正常组比较 ,差异无显著性 (P >0 .0 5 ) ,但Ⅰ组疗效与Ⅱ组比较 ,差异有显著性 (P <0 .0 5 )。结论 IBD发病与免疫功能低下有关 。

Objective To discuss the pathogenic mechanism and observe the effect on T lymphocyte adhesion molecule in animal model of inflammatory bowel disease (IBD).Methods Thirty SD white rats were used to make IBD models by immunological method,and they were divided into: groupⅠ,treated with herbal enemas( n =10);groupⅡ,treated with SASP enemas( n =10);group Ⅲ, treated with normal saline enemas( n =10);and their levels of LTR,IL?2,CD4 + ,CD8 +,CD4 +CD11a +,CD4 +CD18 +,CD8+CD11a +,CD8 +CD18 + were tested by cellular molecular assay method.Results The levels of LTR,IL-2,CD8 +,CD8 +CD11a +,CD8 +CD18 + in model Group Ⅲ were lower than those in the non model normal group( P <0.01).After being treated with different enemas,the levels of LTR,IL?2,CD8 +,CD8 +CD11a +,CD8 +CD18 + were raised in groupⅠand group Ⅱ,but the effect of groupⅠwas better than groupⅡ( P < 0.05 ).Conclusion The lowering of immunity function is the pathogenic mechanism of IBD.The herbal enema treatment on IBD can raise the lower level of immity function.