锌带蛋白基因ZNRD1高表达胃癌细胞模型的建立

ESTABLISHMENT OF A GASTRIC CANCER CELL MODEL WITH HIGH EXPRESSION OF ZINC RIBBON PROTEIN GENE ZNRD1 BY MICROINJECTION

为建立高表达ZNRD1胃癌细胞模型 ,通过分子克隆技术将ZNRD1基因全长cDNA片段克隆入真核表达载体pcDNA3 1+的多克隆位点之间 ,对重组质粒进行酶切鉴定 ,经显微注射将重组质粒导入人胃癌细胞SGC790 1,G4 18筛选后应用Northernblot检测ZNRD1基因的表达。结果经酶切鉴定证实正确地构建了真核表达质粒 ,转染SGC790 1细胞后获得有效表达。表明已成功地构建了ZN RD1基因真核表达载体 ,并经显微注射法建立了高表达锌带蛋白基因ZNRD1的胃癌细胞模型 ,为深入研究ZNRD1的作用和机制奠定了基础。

To construct a eukaryotic vector and establish a gastric cancer cell model highly expressing zinc ribbon protein gene ZNRD1, ZNRD1 cDNA was inserted into multiple cloning sites of the pcDNA3 1 + with molecular cloning technique. The recombinant vector was identified by endonuclease digestion and transfected into SGC7901 cells by nucleus microinjection. Northern blot was used to detect the expression of ZNRD1 in cells. The results showed that ZNRD1 was successfully cloned into pcDNA3 1 + and microinjected into SGC7901. Therefore, a gastric cancer cell model highly expressing ZNRD1 has been established and can be used for further functional research of this gene.