高压氧对脊髓损伤大鼠脊髓组织中兴奋性氨基酸的影响

EFFECT OF HYPERBARIC OXYGEN ON CONCENTRATION OF EXCITATORY AMINO ACIDS IN SPINAL CORD TISSUES OF RATS WITH SPINAL CORD INJURY

通过复制脊髓损伤(SCI)模型 ,用常氧、高压氧(HBO)、氮氧混合气、激素等分别处理各组大鼠后 ,取脊髓组织测兴奋性氨基酸(EAA ,包括天冬氨酸Asp及谷氨酸Glu)含量 ,以探讨HBO治疗SCI的机制。结果显示 ,损伤后 ,大鼠脊髓组织中EAA(Glu)含量明显升高 ,经不同处理后 ,除氮氧混合气组外 ,Glu含量均有所下降 ,以 0 2 5MPaHBO组和 0 2 5MPaHBO +激素组下降最明显 ,与损伤组比较有显著性差异 (P <0 0 1) ,与正常对照组比较则无显著性差异 ;0 2 5MPaHBO组与 0 1MPaHBO组比较及 0 2 5MPaHBO +激素组与激素组比较 ,均有显著性差异 (P <0 0 1)。提示HBO能通过减少组织中的EAA减轻SCI后的继发性损伤 ,恢复脊髓组织的功能 ,其中以0 2 5MPaHBO和 0 2

To study the mechanism of hyperbaric oxygen (HBO) on spinal cord injury (SCI), we duplicated a SCI model and determined the concentrations of excitatory amino acids [EAA, including aspartic acid (Asp) and glutamic acid (Glu)] in spinal cord tissue after treated with 0 1 MPa HBO, 0 25 MPa HBO, 0 25 MPa N 2 O 2 and hormone, respectively. The results showed that the concentration of EAA (Glu) in the spinal cord significantly increased after injury, whereas after treatment it decreased in all but the N 2 O 2 group. It decreased most significantly in 0 25MPa HBO and 0 25MPa HBO+hormone groups. There were significant differences between 0 25MPa HBO group and 0 1MPa HBO group, 0 25MPa HBO+hormone group and pure hormone group ( P <0 01). These results suggest that HBO can recover the function of spinal cord tissues throught decreasing EAA in tissues and thus relieve the secondary injury of SCI. The best treatment is using 0 25MPa HBO and 0 25MPa HBO+hormone.