高温α-淀粉酶产生菌株的选育

The Screening of A Thermostable α-Amylase-Producing Strain

从西藏当雄温泉附近的土壤中,分离到一株能在55℃生长并分泌高温α 淀粉酶的菌株,经初步鉴定为凝结芽孢杆菌,命名为BacilluscoagulansLS 1.该菌株用紫外线和亚硝基胍诱变,在55℃摇瓶培养条件下,筛选到一个发酵液酶活达到100U/mL的突变株,较出发菌株的酶活提高了约25倍.发酵液经90℃处理60min,酶活性保持在90%以上.

A stain of thermostable αamylaseproducing bacteria was screened from soil samples of Dangxiong hot spring,Tibet.It is identificated as Bacillus coagulans.The strain was treated by UV and NTG,the thermostable αamylase activity was raised 25 times,100 U/mL,in the shake flask at 55℃.It was stable by heating at 90℃ for 60 min.