抗敏胶囊主要成分的HPLC法分析

Analysis of the Main Ingredients in Anti-allergy Capsules by HPLC

目的 采用高效液相色谱法分析抗敏胶囊中主要成分（黄芩苷和淫羊霍苷）。方法 测定黄芩苷,以Diamosil C18柱（250mm×4.6mm）为色谱柱;0.05mol/L磷酸水溶液-乙腈（70:30）为流动相;流速1ml/min;检测波长277nm;测定淫羊霍苷,以Spherisorb C18（250mm×4.6mm）为色谱柱;乙腈-水-冰醋酸（22:78:0.1）为流动相;流速1ml/min;检测波长270nm。结果 黄芩苷的平均回收率为99.2％,淫羊霍苷的平均回收率为100.4％,符合分析要求,重复性试验的RSD分别为:黄芩苷0.84％,淫羊霍苷1.6％。结论 重复性试验结果良好。另外还对样品的提取条件和测定条件进行了优选,为评价含黄芩苷和淫羊霍苷类药材及其制剂的质量提供了一个灵敏、准确和快速的测定方法。

AIM To develop HPLC method for the determination of the main ingredients (Baicalin and Icariin) in Anti-allergy Capsules. METHODS The measurement conditions used as follows; For Baicalin, Diamosil C18 Column; mobile phase: 0. 05mol/L phosphate solution-Acetonitrile (70: 30) ; flow rate 1 ml/min; wavelength for measurement 277 nm;For Icariin, Spherisorb C18 column; mobile phase: Acetonitrile-water-acetate acid(22:78: 0. 1); flow rate 1 ml/min; wavelength for measurement 270 nm. The recovery rates for Baicalin and Icariin were 99. 2% and 100. 4% respectively and RSD of them were 0. 84% and 1. 6% respectively. CONCLUSION The procedure is simple and rapid. The results are stable and reproducible. The method can be used for evaluation of crude drugs and their preparations containing Baicalin and Icariin.