蛇葡萄素的抑菌作用研究

Studies on Antibacterial Activity of Ampelopsin

目的 对瑶族藤茶中主要成分蛇葡萄素的体外抗菌活性进行评价。方法 以两倍稀释法测定了蛇葡萄素对五个实验菌株的抑菌效果,并与相同实验条件下盐酸黄连素的抑菌效果相比较,另外还研究了培养基pH值和菌液浓度对蛇葡萄素抑菌作用的影响。结果 蛇葡萄素对实验菌株有较好的抑菌作用,其抑菌作用较盐酸黄连素相当或略强。菌液浓度增高,其MIC和MBC随之增高,对试验菌株,酸性、碱性条件下蛇葡萄素的抑菌作用显著增强。

AIM To evaluate the antibacterial activity of ampilopsin from Ampelopsis grossedentata(Heind-Mazz) W. T. wang in vitro. METHOD MIC determination of ampelopsin antibacterial which was used in experiment was performed by the tube double dilution method, and MBC was performd by plate coating method. The MIC and MBC of each bacterium were compared with berberine hydrochloride which was tested under the same condition. In addition, the influence on the activity of ampelopsin by pH condition of culture medium and the concentration of liquid bacterial germ was investigated. RESULTS Ampelopsin has stronger antibacterial activity than that of berberine hydrochloride. The MIC and MBC of ampilopsin were increased with the concentration of liquid bacterial germ, and were decreased when the pH of culture medium was under acidic or alkaline condition.