原子力显微镜对泌尿系结石超微结构的研究

Study on the ultrastructure of the urinary stone in atomic force microscopy

目的 研究和观察泌尿系结石的超微结构。方法 10枚结石分别做超薄切片(厚度小于3mm),打磨抛光处理,并收集粉末。先行原子力显微镜观察,再做成电镜标本观察分析。结石粉末均行X-线衍射检测。结果 得到肾盂结石和膀胱结石超微结构的AFM图像和电镜扫描图像,X-线衍射检测有7枚是草酸钙结石,3枚是磷酸钙结石。测得草酸钙结石和磷酸钙结石的微小晶粒尺寸。分析AFM的反转片信息,获得结石基质的空间构架图像。结论 应用原子力显微镜技术可以实现对泌尿系结石超微结构直接实时的观察分析。尿结石的超微结构形态与结石所处部位无关,而取决于结石晶体类型。草酸钙结石的晶体和基质的AFM图像与磷酸钙结石不尽相同,晶体尺寸有差异(P<0.05)。结石基质的空间构架为晶体的聚集提供了可能性。

Objective To study the wtrastructure of the urinary stone croscopy. Methods We selected ten samples from the pyelolithotomy and the lithntomy of urinary bladder, which were polished and made imo ultrathin sections, simultaneously the powder were collected. All of the samples observed in SEU and AFM and the powder were examined by X - ray diffraction. Resells The AFM images of the pelvi - calculus and the bladder calculus were obtained. The minute crystal dimension of the calcium phosphate stone and the calcium oxalate stone were measured, and the space configurations of the stone matrix were suspected. Conclusion The AFM was a good method to observe and analyze the ultrastructurc of the urinary stone directly and real-timely. The specialty of the ultrastructure was due to the crystal components not the locus of the stone. The calcium phosphate crystal could be differentiated from the calcium oxalate according to the AFM images and their differ dimensions. The space configurations of the matrix may prompt the accumulation of the stone crystals.