贲门失弛缓症犬动物模型的建立

Establishment of achalasia model in dogs

目的:应用苄基二甲基十四烷氯化铵(benzyldimethyltetrade-cylammoniumchloride,BAC)建立犬贲门失弛缓症动物模型.方法:将BAC4mmol/L12mL在胃镜下环周注入犬下食管括约肌(LES),用生理盐水作正常对照,注射6wk后测定LES的压力以及LES的松弛度.观察两组动物临床表现,食管吞钡时食管钡剂潴留情况.应用免疫组化(ABC法)染色LES组织观察NOS神经元的差异.结果:BAC处理组LES压力显著高于生理盐水组(5.66±0.56kpavs3.03±0.69kpa,P<0.01),LES的松弛率和松弛度明显低于生理盐水组(松弛率:40%vs100%;松弛度:13.1±3.9%vs94.3±3.4%),差别十分显著(P<0.01),反食为60%vs0%(P<0.01)质量减轻1.1±0.5kgvs-0.5±0.2kg(P<0.05).食管吞钡显示,BAC处理组食管钡剂明显潴留,而生理盐水组正常.免疫组化染色证实BAC处理组NOS阳性神经元明显减少.结论:LES局部应用BAC,能有效建立贲门失弛缓症犬动物模型.

AIM:To develop an achalasia model in dog.METHODS:Benzyl-dimethyltetradecylammonium chloride(BAC)(4 mmol/L, 12 mL) was endoscopically injectedcircumferentially into the lower esophageal sphincter(LES)in 10 adult dogs and saline injection in 10 adult dogs ascontrol. Six weeks after injection, manometry was performed,and the frequency and degree of relaxation were recorded.The clinical symptoms and esophageal barium test wereobserved. By means of immunochemical method, NOS-posi-tive myoenteric ganglion cells were stained.RESULTS:The LES pressures (LESP) in BAC-treated dogswere significantly higher than that in saline control (5.66±0.56 kpa vs 3.03±0.69 kpa).The frequency and degree ofLES relaxation in BAC-treated dogs were lower(relaxationfrequency: 40 % vs.100 %;relaxation degree:13.1 ± 3.9 %vs. 94.3 ± 3.4 %). Regurgitation and weight loss wereaccordingly 60 % vs 0 % (P <0.01) and 1.1±0.5 kg vs-0.5 ± 0.2 kg (P <0.05). The difference of symptoms wasvery significant compared with that in saline control. Esoph-ageal barium retention was founded in BAC-treated dogsbut normal in saline control. The BAC-treated tissue showeda marked loss of myenteric neurons by means of immunohis-tochemistry.CONCLUSION:Achalasia model in dogs can be establishedby injection of BAC into LES.