阿魏酸钠对大鼠晶状体上皮细胞凋亡相关基因bcl-2和bax的调控

Effect of sodium ferulate on expression of bcl-2 and bax in rat lens epithelial cells

目的 :本研究旨在探讨阿魏酸钠 (SF)对实验性氧化损伤大鼠晶状体上皮细胞 (LEC)凋亡相关基因bcl- 2和bax的调控。方法 :将SD大鼠双眼随机分成空白对照组、过氧化氢 (H2 O2 )组、吡诺克辛 (PS)组和SF组。无菌操作摘除眼球并在手术显微镜下分离晶状体 ,使晶状体孵育在 30 0 μmol·L- 1 H2 O2 培养液中复制LEC凋亡模型 ,同时加入终浓度为 5mmol·L- 1 的SF ,置二氧化碳培养箱共同孵育 2 4h。取晶状体前囊膜采用免疫组化法检测凋亡相关基因bcl- 2和bax的蛋白表达并进行比较。结果 :(1 )正常SD大鼠LEC中Bcl- 2和Bax均有表达 ,Bcl- 2表达较Bax表达强。 (2 )H2 O2 组Bcl- 2表达显著下调 ,Bax表达显著上调 (Ridit检验 ,P <0 0 1 ) ;Bcl - 2 /Bax比率下降。(3)与H2 O2 组比较 ,SF可明显上调Bcl- 2表达 ,下调Bax表达 (P <0 0 1 ) ,Bcl- 2 /Bax比率上升。 (4)PS与SF的调节作用相似 ,但SF的作用强于PS(P <0 0 5)。结论 :本研究结果表明 ,SF调控凋亡相关基因bcl-

AIM: To investigate the effect of sodium ferulate on expression of apoptosis-related genes, bcl-2 and bax , in rat lens epithelial cells (LEC) injured by oxidation.METHODS: Eyes of SD rats were divided randomly into four groups: control group, hydrogen peroxide group (H 2O 2), pirenoxine sodium group (PS) and sodium ferulate group (SF). Eyes were excised and lenses were separated under operating microscope and sterilized condition. Lenses were incubated in CO 2 incubator for 24 h with 300 μmol·L -1 H 2O 2 and with or rithout 5 mmol·L -1 SF. The expression of Bcl-2 and Bax protein of LEC were measured and compared by tearing the LEC anterior capsule via immunohistochemical analysis.RESULTS: (1) There were Bcl-2 and Bax expression in normal lenses of SD rates, Bcl-2 expression was stronger than Bax. (2) Bcl-2 expression decreased and Bax expression increased markedly ( P<0 01 ),Bcl-2/Bax reduced in H 2O 2 group. (3) There were up regulation of Bcl-2 expression and down-regulation down of Bax expression, and Bcl-2/Bax increased in SF group compared with H 2O 2 group ( P<0 01 ). (4) The above changes were similar between PS group and SF group, but much stronger in SF group than that in PS group ( P<0 01 ).CONCLUSION: These results show that SF regulates expression of apoptosis-related genes, bcl-2 and bax , which may be the molecular mechanism of LEC apoptosis inhibition by SF.