简便法分离回收血浆白蛋白并清除胆红素

Easy method for separation and recovery of albumin and clearance of bilirubin from plasma

在用人工肝治疗时 ,患者要丢失大量血浆。为了分离回收人工肝废弃血浆中的白蛋白 ,我们用中性盐NaCl盐析法沉淀分离白蛋白 ,再用碱性活性碳清除与白蛋白结合的胆红素 ,可回收血浆中 83%的白蛋白 ,清除血浆中 74 %的胆红素 ;回收的白蛋白溶液中NaCl浓度比回收前增高 1 5 - 2倍 ,HBVDNA载量降至用PCR荧光定量法测不出。本法简便安全 ,回收的血浆白蛋白没有变性 。

During the treatment of artificial liver, a lot of plasma of the patient will be discharged. To separate and recover the albumin from the discharged plasma, we developed the NaCl salt-out and the basic activated carbon adsorbtion methods which showed a rate of recovery of 87% for albumin and a clearance rate of 74% for bilirubin. In the recovered albumin solution, NaCl cocentration was increased by 1 5-2 folds and the load of HBV DNA was undetectable by fluorogenic quantitative PCR. The new method was easy and safe. The recovered albumin has not been denatured. It's very possible that the recovered albumin to be transfused back to the patient in the future.