转录因子E2F在前列腺增生组织中表达的意义

The Significance of transcription factor E2F expression in benign prostatic hyperplasia

目的 探讨细胞周期转录因子E2F在良性前列腺增生症 (BPH)发病过程中作用。方法 应用免疫组化方法检测E2F、细胞周期蛋白E(cyclinE)、cyclinD1和PCNA在 62例BPH组织和 14例正常前列腺 (NP)组织中的表达 ;应用DNA图像定量分析法测定BPH组织中DNA含量和细胞周期不同时相细胞比率。结果 E2F表达主要位于前列腺上皮细胞 ,BPH中阳性率为 66.1% ,显著高于NP的 2 8.6% (P <0 .0 1)。BPH组织中E2F阳性表达者的G0 /G1期细胞比率较阴性表达者呈降低趋势 (P =0 .0 5 1)、而S期细胞比率、DNA含量和PCNA指数显著高于阴性表达者 (P <0 .0 5 )。BPH组织中E2F表达与cyclinE表达之间密切相关。 结论 E2F异常高表达导致细胞周期紊乱、细胞增殖增加 。

Objective To investigate the role of transcription factor E2F in the pathogenesis of benign prostatic hyperplasia(BPH).Methods The expressions of E2F,cyclin E,cyclin D1 and PCNA in 62 cases with BPH and 14 normal prostatic tissues (NP) were examined by immunohistochemistry.DNA parameters in BPH were determined by DNA image cytometry.Results Positive stains of E2F mainly located in epithelium of prostate.The E2F positive expression was found in 66.1% of BPH,which was significantly higher than 28.6% of NP.G0/G1 phase fraction in E2F positive expression of BPH was tendency to lower than E2F negative expression,while S-phase fraction,DNA content and PCNA index in E2F positive expression was significantly higher than in E2F negative expression.There was a close correation between E2F expression and cyclin E expression in BPH.Conclusion Abnormal expression of E2F play an important role in development and progresion of BPH.