乙型肝炎病毒感染者前C区A83位点突变

Analysis of precore A83 mutations in serum of patients infected by hepatitis B virus

目的 分析乙型肝炎病毒 (HBV)感染者前C区A83位点突变情况及其与HBeAg表达的关系。 方法 对 12 2例乙型病毒感染者采用Amplificationrefactorymutationssystem (ARMS)方法进行检测 ,扩增前C区基因片段经过酶联反应显色。 结果 在HBeAg阳性的 5 4例患者血清中检测出 5例A83位点突变 ,占 9.3% ,在HBeAg阴性的 6 8例患者血清中检测出 4 8例A83位点突变 ,占 70 .6 %。 15例急性乙型肝炎 (AH)患者未检测到A83位点突变 ,70例慢性乙型肝炎患者 (CH)检测到 2 6例A83位点突变 (35 .6 % ) ,2 9例乙肝肝硬化患者 (HLC)中有 19例A83位点突变 (6 5 .5 % ) ,8例乙肝肝癌患者 (HCC)均有A83位点突变 (10 0 % )。 结论 HBeAg阴性患者A83位点突变率高于HBeAg阳性患者 。

Objective To analyse the A83 mutations in serum of persons infected by hepatitis B virus,and to explore the relationship between precore A 83 mutation and express of HBeAg. Methods Amplification refactory mutations system were used to analysis of precore A83 mutations in serum of 122 infected persons with hepatitis B virus. Results Precore A83 mutations had detected in serum of 5 cases among 54 persons with HBeAg positive(9.3%), and 53 cases among 68 persons with HBeAg neagitive. Precore A83 mutation had also detected in all patients (8 cases) with hepatocellular carcinoma, 26 cases among 70 patients with chronic hepatitis (35.6%),and 19 cases among 29 patients with hepatitis liver cirrhosis (65.5%). There was no precore A83 mutations in 15 patients with acute hepatitis. Conclusion The rate of precore A83 mutations in serum of persons with HBeAg negative is higher than persons with HBeAg positive.Precore A83 mutations may be associated with chronic changes after infection of hepatits B virus.