兔睾丸扭转复位后睾丸细胞凋亡的观察

Testicular apoptosis after torsion reduction

目的 观察兔睾丸扭转复位后睾丸细胞凋亡情况及药物减轻睾丸凋亡的效果。方法 选用青春期雄性日本大耳白兔 2 0只 ,体重 90 0～ 110 0g ,月龄 2～ 3个月 ,随机分为 4组 ,每组 5只。手术制作幼兔睾丸扭转模型 (72 0° ,2h) ,对部分扭转组应用抗氧化剂 (抗坏血酸 )或钙离子通道拮抗剂 (维拉帕米 )。 2d后取出睾丸 ,TUNEL法观查各组动物睾丸组织细胞凋亡情况 ,HE染色检查睾丸病理组织学改变。结果 幼兔睾丸扭转复位后 ,精原细胞发生凋亡 ,而睾丸支持细胞及间质细胞未见凋亡现象。单纯扭转组精原细胞平均凋亡指数 (AI) (7.92± 1.2 9)‰ ,较对照组 (2 .6 0± 1.0 1)‰显著升高 ;睾丸扭转前后应用抗坏血酸及维拉帕米组的睾丸细胞平均AI ,分别为 (4.12± 0 .73)‰ ,(4.0 8± 0 .88)‰ ,较单纯扭转组 (7.92± 1.2 9)‰显著下降。HE染色的组织学改变与TUNEL法类似。结论 睾丸扭转损伤后可致使生精细胞凋亡。

Objective To assess the apoptosis of rabbit bunny testes after experimental testicular torsion and the effect of medical rescue. Methods A model of 20 Japanese bunny rabbits (weight 900～1100?Kg) of testicular torsion (720 degree, 2hrs) was created. The rabbits were divided into 4 groups at random (5 for each ).Antioxidant and Ca 2+ channel blocker were administrated in the intervention groups. The apoptosis was assessed by TUNEL technique and tissue pathology was examined by hemotoxylin and eosin staining. The results from each group were compared. Results Ischemia reperfusion injuries occurred after testicular torsion. The mean apoptosis index (AI) in torsion group was ( 7.92 ± 1.29 )‰, which was significantly higher than that of the control group ( 2.60 ± 1.01 )‰. The administration of antioxidant and Ca 2+ channel blocker can significantly reduce AI ( 4.12 ± 0.73 )‰,( 4.08 ± 0.88 )‰, respectively. Conclusions The spermatogonium apoptosis increases significantly as result of testicular torsion. The administration of antioxidant and Ca 2+ channel blocker alleviate the apoptosis.