耳针、中药对糖尿病大鼠CGRP、ET、TXB_2及6-Keto-PGF_(1α)影响的实验研究

Experimental Study of Effect of Ear Acupuncture and Chinese Medical Herbs on CGRP, ET, TXB2 & 6-Keto-PGF1a,of Diabetic Rats

目的:研究耳针、中药对糖尿病大鼠血浆降钙素基因相关肽(Calcitonin gene relatedpeptide,CGRP)、内皮素(Endothelin,ET)、血栓素B_2(Thromboxand B_2,TXB_2)及6-酮-前列腺素F_(1α)(6-Keto-PGF_(1α))等的影响。方法:利用链脲佐菌素(STZ)诱发的糖尿病大鼠接受耳针、中药治疗14周后,放免法测定血浆CGRP、ET、TXB_2及6-Keto-PGF_(1α)等指标。结果:与正常组相比糖尿病大鼠血浆CGRP、ET及TXB_2水平明显升高,6-Keto-PGF_(1α)水平稍有降低,E/C及T/P明显升高,而耳针、中药治疗后均明显改善。提示:耳针、中药防治糖尿病微血管病变的一种可能机制是良性调整血浆ET与CGRP及TXB_2与6-Keto-PGF_(1α)水平。

Purpose: To investigate the effect of ear acupuncture and Chinese medical herbs on Calcitonin gene related peptide( CGRP)、Endothelin(ET) .Thromboxand B2(TXB2)and 6-Keto-PGF1a in diabetic rats. Methods: After ear acupuncture and Chinese medical herbs were given to streptozotocin - induced diabetic rats for 14 weeks, radioim-munoassy was used to measure the plasma CGIU、ET、TXB2 & 6 - Keto - PGF1a. Results: CGRP、ET and TXB2 levels were significantly increased and 6 - Keto - PGF1a. lowered slightly in diabetic rats compared with control group. Treatment of ear acupuncture and Chinese medical herbs could obviously reduce levels of plasma CGRP, ET, TXB2. Conclusion: Above results indicate that one possible preventive and therapeutic mechanism of ear acupuncture and Chinese medical herbs in the diabetic microangiopathy was to up - modulate the level of CGRP & ET, TXB2 & 6 - Keto - PGF1a and adjusting the ratio of the former.