期刊文献+

高温对HepA瘤株细胞周期的影响 被引量:9

The effects of hyperthermia on the cell cycle of HepA cells
原文传递
导出
摘要 目的 探讨高温对肿瘤细胞周期的影响。方法 将肝癌腹水型瘤株HepA细胞分为 3 7℃、40℃、42℃和 44℃四个温度组。每一组分别在 1h、2h、4h和 8h四个时间点取材 ,经PI染色在流式细胞仪上检测四种温度条件下 ,四个时间段时HepA细胞在各细胞周期中的细胞数。结果  3 7℃时 ,细胞处于活跃的增殖状态 ,绝大部分细胞处于S及G2 /M期。 40℃时 ,细胞增殖更趋活跃 ,G1期细胞减少。 42℃时 ,细胞增殖受到明显抑制 ,G1期细胞增加 ,G2 /M期细胞减少。 44℃时 ,12h ,细胞增殖受到进一步的抑制 ,G1期细胞明显增加 ,G2 /M期细胞进一步减少 ;48h ,高温对细胞增殖的影响反而减弱 ,与 12h时相比 ,G1期细胞明显减少 ,G2 /M期的细胞有了明显的增加 ,甚至多于 42℃时。结论 温和性高温 (4 2 44℃ )对HepA细胞的增殖活动有明显的抑制作用 。 Objective To study the effects of hyperthermia on the cell cycle of tumor cells. Methods The HepA cells were divided into four groups, and placed respectively in 37℃, 40℃, 42℃ and 44℃. Each group was heated for 1, 2, 4 and 8 hours, and tested by the flow cytometer. Results At 37℃, the cells proliferated actively and most cells were in S phase and G2/M phase. At 40℃, the cells proliferated more actively and the number of cells in G1 phase was decreased. At 42℃, the proliferation of the cells was inhibited. The number of cells in G1 phase was increased and that in G2/M phase decreased. At 44℃ for 1 2 hours, the inhibition effect of hyperthermia on cell proliferation was enhanced. The number of cells in G1 phase was increased and that in G2/M phase decreased further. At 44℃ for 4 8 hours, the inhibition effect of hyperthermia on cell proliferation was weakened. The number of cells in G1 phase were decreased and that in G2/M phase increased markedly than at 44℃ for 1 2 hours. Conclusion The moderate hyperthermia(42 44℃) has a remarkable inhibition effect on proliferation of HepA cells and keeps them arrested in the G1 phase.
出处 《中华物理医学与康复杂志》 CAS CSCD 北大核心 2002年第3期144-146,共3页 Chinese Journal of Physical Medicine and Rehabilitation
基金 国家自然科学基金重点资助项目 (No.598362 4 0 )
关键词 高温 肿瘤 细胞周期 热疗 流式细胞术 Hyperthermia Tumor Cell cycle
  • 相关文献

参考文献1

共引文献4

同被引文献63

  • 1黄建武,黄建华,陈金春,陈积贤,张洁,豆长明.门静脉血K-ras和p53基因突变与胃癌肝转移的关系[J].浙江医学,2007,29(4):307-308. 被引量:4
  • 2陈超敏,何杰.在体生物组织热物性测试[J].生物医学工程学杂志,1997,14(2):188-192. 被引量:5
  • 3汪胰 薛绍白 柳惠图.细胞生物学:第2版[M].北京:北京师范大学出版社,2001.226-299.
  • 4汪堃仁 薛绍白 柳惠图 主编.细胞生物学:第2版[M].北京:北京师范大学出版社,2001.5.226—299.
  • 5[1]Sakaguchi Y, Stephens L C, Makino M et al. Apoptosis in tumors and normal tissues induced by whole body hyperthermia in rats. Cancer Res, 1995, 15:5459
  • 6[2]Wyllie A H. The biology of cell death in tumors. Anticancer Res, 1985, 5:131
  • 7[3]Yonezawa M, Otsuka T, Matsui N et al. Hyperthermia induces apoptosis in malignant fibrous histiocytoma cells in vitro. Iit J Camcer, 1996, 66:347
  • 8[4]Rong Y, Mack P. Apoptosis indued by hyperthermia in Dunn osteosarcoma cell line in vitro. Int J Hyperermia,2000, 16:19
  • 9[5]Nakanoma T, Ueno M, Ohigashi Tet al. Anti-proliferatve effects of heating on the human prostatic cells in culture. Hnm Cell, 1998, 11:67
  • 10[6]Uesugi S, Yamashita K, Nakashima K et al. Apoptotic cell death induced by local brain hyperthermia in a rat glioma model. Acta Neuropathol (Berl), 1998, 96:351

引证文献9

二级引证文献27

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部