摘要
目的探讨血啉甲醚对金黄色葡萄球菌的杀伤机制。方法通过原子力显微镜观察不同浓度血啉甲醚作用金黄色葡萄球菌后的形貌变化。结果血啉甲醚对金黄色葡萄球菌有较强的光动力杀伤作用,浓度越高,杀伤作用越强。结论血啉甲醚对金黄色葡萄球菌的光动力杀伤机制是血啉甲醚通过氧化作用攻击细胞结构,造成金黄色葡萄球菌细胞膜破裂,细胞内容物大量外泄,从而导致细菌死亡。
Objective To study the Mechanism of photodynamic inactivation of Staphylococcus aureus(S. aureus) by hematoporyrin monomethyl ether(HMME). Methods The morphological changes of Staphylococcus aureus incubated with different concentrations of HMME were observed by atomic force microscope(AFM). Results S. aureus was very sensitive to the pho todynamic inactivation of HMME, the killing effect was stronger with the concentration increasing. Conclusion Photodynamic Inactivation Mechanism of HMME on S. aureus is the attacktion to the structure by oxidation, so S. aureus is photoinactivated with damages of its membrane and its contents leakage from the cell.
出处
《解剖学研究》
CAS
2014年第4期285-287,291,共4页
Anatomy Research
关键词
血啉甲醚
金黄色葡萄球菌
原子力显微镜
Hematoporyrin monomethyl ether
Staphylococcus aureus
Atomic force microscope
