卵白蛋白致敏降低新生期小鼠神经发生和学习记忆功能

OVA immunization impairs neurogenesis and learning and memory function in neonatal mice

目的研究卵白蛋白(OVA)引起的Th1/Th2平衡偏移对新生小鼠学习记忆功能和神经发生的影响,及将其应用于神经免疫相关实验研究的可行性。方法将新生C57BJ/6小鼠随机分为OVA处理组和PBS对照组。用卵白蛋白(OVA)塑造Th1/Th2平衡偏移小鼠模型。水迷宫实验检测小鼠海马依赖的学习记忆功能;ELISA法测血清和海马组织的IFN-γ,IL-4以及海马的BDNF水平;免疫荧光组化检测小鼠的神经发生。结果 OVA组小鼠学习记忆能力、神经发生及海马BDNF水平低于PBS组,差异有统计学意义(P<0.05);新生小鼠血清和海马的IFN-γ和IL-4浓度低于对照组(P<0.01),且OVA组小鼠血清和海马中IFN-γ/IL-4比值较对照组明显降低,差异均具有统计学意义(P<0.05)。结论 OVA致敏导致新生小鼠Th1/Th2平衡向Th2偏移,削弱了其学习记忆功能,降低了小鼠的神经发生;OVA不适合作为神经免疫相关研究实验中的空白对照。

Objective To study effect of the shift of Th1/Th2 balance induced byovalbumin immunization on neurogenesis and cognitive function in neonatal mice,and feasibility of application of it to neuroimmunology research. Methods Neonatal C57BL/6 mice were randomly assigned to OVA group and PBS group. OVA were injected to remodel Th1/Th2 balance in neonatal mice. Learning and memory function was tested by Morris water maze. ELSA was performed to detect levels of IFN- γand IL-4in serum and hippocampus and hippocampal BDNF level. Neurogenesis was measured by immunofluorescence. Results Learning and memory function,neurogenesis and hippocampal BDNF in OVA group was decreased when compared with PBS group(P<0.05).OVA group shows significantly lower levels of IFN- γ and IL-4 in serum(P<0.01)and hippocampus than PBS group(P<0.05).There was a higher ratio of IFN-γ to IL-4 in OVA group compared with PBS group in serum and hippocampus(P<0.05). Conclusion OVA immunization induces shift of Th1/Th2 to Th2,which impairs learning and memory function and decreases neurogenesis of neonatal mice. OVA can not be taken as blank control of neuroimmunology research。