慢性乙型肝炎患者HBV病毒载量与髓过氧化物酶检测分析

Analysis between HBV-DNA viral load,Myeloperoxidase in serum of chronic hepatitis B patients

目的探讨慢性乙型肝炎(CHB)患者血清中HBV-DNA载量与髓过氧化物酶(MPO)及超敏C反应蛋白(hsCRP)水平的相关性。方法对121例慢性乙肝患者的血清采用荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA载量,免疫透射比浊法分别测定血清MPO和hs-CRP的水平。结果 CHB患者MPO、hs-CRP水平显著高于正常对照组(P均<0.01),且随肝损害程度的加重而升高(P<0.01或P<0.05);不同HBV载量组间MPO及hs-CRP水平比较,差异均无统计学意义(P>0.05)。hs-CRP及MPO水平变化与HBV-DNA载量均无相关性(r=0.254,r=0.752,P>0.05)。结论 MPO可作为评价CHB患者炎症反应的指标,多数CHB患者具有不同程度炎症反应,但其炎症程度与乙肝病毒复制程度无关。

Objective To investigate the relationship between HBV-DNA viral load,Myeloperoxidase and high sensitive C-reactive protein( hs-CRP) in serum of chronic hepatitis B( CHB) patients. Methods Immune transmission nephelometry and rate method were used to measure the levels of MPO、and hs-CRP in serum of 124 patients with CHB,HBV-DNA in serum were determined by FQPCR. Results Serum MPO and hs-CRP levels of CHB were higher obviously than in normal control group,and were correlated with degree of liver damage positively( P < 0. 01 or P < 0. 05). No significant differences were observed in levels of hs-CRP and between HBV DNA high / low groups( P > 0. 05). No correlation was found between the levels of hs-CRP and MPO and viral load of HBV-DNA( r = 0. 254,r = 0. 752,P > 0. 05). Conclusion MPO could be a useful indicator for evaluating inflammatory of chronic HBV carriers. Most chronic HBV carriers had variously inflammatory histological changes in their livers,but the grades of liver inflammation were not correlated with HBV-DNA in serum.