EpCAM、Oct-4在肝细胞癌中的表达及临床意义

Expressions and clinical significance of EpCAM and Oct-4 in hepatocellular carcinoma

目的探讨干细胞表面标志物Ep CAM和Oct-4在肝细胞癌中的表达分布情况及其与肝细胞癌的关系。方法应用免疫组化S-P法检测65例肝细胞癌和相应癌旁组织中Ep CAM和Oct-4的表达、分布情况。结果肝细胞癌组织中Ep CAM和Oct-4蛋白产物阳性表达率分别为43.1%(28/65)和53.8%(35/65)(P<0.05),明显高于相应癌旁组织4.62%(3/65)和7.69%(5/65),Ep CAM在高水平AFP组的肝细胞癌患者中呈高表达(P<0.05),而Oct-4在无肿瘤包膜组或有门静脉癌栓组的肝细胞癌患者中呈高表达(均P<0.05),Ep CAM与Oct-4在肝细胞癌中的表达呈正相关(P<0.05)。结论 Ep CAM和Oct-4可能与肝细胞癌的发生、发展相关,Oct-4有可能成为肝癌干细胞表面标记物。

Objective To investigate the expression of Ep CAM and Oct-4 in hepatocellular carcinoma tissues and explore the relationship with hepatocellular carcinoma. Methods The histological and immunohistochemical features of 65 cases of hepatocellular carcinoma and paraneoplastic liver tissues were examined by immunohistochemistry SP methods to investigate the expression and distribution of Ep CAM and Oct-4 positive cells. Results The positive rates of Ep CAM and Oct-4 expression were significantly higher in hepatocellular carcinoma than in paraneoplastic liver tissues( 43. 1% vs 4. 62%,53. 8% vs 7. 69%)( P < 0. 05),Ep CAM expression was positively correlation with AFP level( P < 0. 05),and the level of Oct-4 expression was related with tumor capsule or portal vein tumor thrombus,the positive rates of Oct-4 was significantly higher in the hepatocellular carcinoma with portal vein tumor thrombus or without tumor capsule( P < 0. 05). The expression of Ep CAM was positively correlated with that of Oct-4( P < 0. 05). Conclusion Ep CAM and Oct-4 expressions correlates positively with the progression of hepatocellular carcinoma,Oct-4 may be regarded as potential marker of liver cance stem cell.