黄芪甲苷调节成纤维细胞功能活性的作用研究

Effect of astragaloside Ⅳ on the functional activities of fibroblasts in vitro

目的研究黄芪甲苷对成纤维细胞增殖、迁移和胶原蛋白生成的影响,为黄芪甲苷在组织创面愈合中应用奠定基础。方法体外培养小鼠成纤维NIH 3T3细胞,观察黄芪甲苷处理前后NIH3T3细胞功能变化。采用MTT法和流式细胞术检测细胞增殖情况,Transwell小室和细胞划痕实验检测细胞迁移能力,real-time PCR测定细胞外基质相关蛋白基因表达水平。结果与PBS对照组比较,5μg/m L黄芪甲苷处理明显增加NIH3T3细胞的增殖活性,促进细胞的迁移能力和细胞划痕的创伤愈合速率(P<0.05)。同时,黄芪甲苷处理上调细胞外基质相关基因CollagenⅠ、CollagenⅢα、Fibronectin的表达(P<0.05)。结论黄芪甲苷通过多途径调节成纤维细胞的功能活性,为明确其在创面愈合中的作用提供了依据。

Objective To investigate the effect of astragalosideⅣon the proliferation,migration and collagen production of fibroblasts,and provide a reference for application of astragalosideⅣin wound healing.Methods Mouse NIH 3T3 fibroblasts were cultured in vitro,and the functional activities of NIH3T3 cells were measured before and after treatment with astragalosideⅣ.MTT assay and flow cytometry were used to detect cell proliferation,transwell chamber and cell scratch assay were used to detect cell migration ability,and real-time PCR was used to detect the expression level of extracellular matrix-related protein genes.Results Compared with the control groups,5μg/m L astragalosideⅣfacilitated the proliferation of NIH 3T3 cells,promoted cell migration and cell scratch healing.Meantime,astragalosideⅣenhanced the expression of extracellular matrix-related genes Collagen I,CollagenⅢαand Fibronectin.Conclusion AstragalosideⅣmultiply modulates the functional activity of fibroblasts,which may be involved in the wound healing.